ABSTRACT The use of cover crops and poultry manure (PM) is an alternative to reduce the use of synthetic inputs and can contribute to the nutrient cycling in onions ( Allium cepa L.) grown under a no-tillage system. However, this management practice may contribute to an increase in N2O emissions to the atmosphere. The aims of this study were to evaluate the immediate effect on N2O emissions of adding PM onto cover crop residues and to verify the effect of different no-tillage systems on N2O emissions. Two studies (laboratory and field) were conducted with the addition of oilseed radish (OR), black oat (BO), and weed (WD) residues with and without PM under a no-tillage (NT) system. Emission of N2O (kg ha-1) was influenced by the different residue-management systems and was higher in treatments with OR residues (2.96 ± 0.67 kg ha-1 for OR and 5.28 ± 1.04 kg ha-1 for OR + PM). The other treatments behaved similarly with emissions of approximately 1.91 ± 0.17 kg ha-1 of N-N2O. The highest N2O emissions in the field study were found within the first 15 days and represented 50.3 % of the average emissions. Poultry manure showed high emissions when the cover crop was OR, but not when it was BO and WD.

ABSTRACT Sandy soils of the Campanha Gaúcha region have naturally low levels of available phosphorus (P), which is why special attention to pre-planting fertilization is important. However, there are no studies that enable a proper calibration method to determine P content available in soil for young grapevines. The study aimed to evaluate phosphorus fertilization for young grapevines of Chardonnay and Pinot Noir in order to provide support for the recommendation of P in sandy soils. In October, 2011 we applied six doses of P (0, 10, 20, 40, 60 and 100 kg P2O5 ha-1 yr-1) in soil and transplanted cultivars of Chardonnay (experiment 1) and Pinot Noir (ex-periment 2). The design of each experiment was randomized blocks with ten plants and three replications. We evaluated P content in the soil and leaves. We determined plant height, stem diameter and dry matter of the pruned material from crop seasons 2011/12 to 2014/15. The addition of increasing P doses elevated P content available in soil three years after implementing the experiments, but exclusively in doses above 40 and 60 kg P2O5 ha-1 in Pinot Noir and Chardonnay, respectively. However, P content in soil for every applied P dose remained below sufficiency level, and therefore growth parameters and leaf P content were virtually unaffected.

RESUMEN Los suelos arenosos de la región Campanha Gaúcha son naturalmente bajos en contenidos de fósforo (P) disponible, por lo que requiere una atención especial con la fertilización previo a la siembra. Sin embargo, aún no existen trabajos de investigación que permitan una calibración adecuada para determinar el contenido de P disponible en suelos para el cultivo de vides viníferas jóvenes. El objetivo de esta investigación fue evaluar la fertilización fosfatada en las variedades jóvenes de vid Chardonnay y Pinot Noir a fin de sugerir dosis a la recomendación de P, en suelos arenosos. En Octubre de 2011 fueron aplicadas seis dosis de P (0, 10, 20, 40, 60 y 100 kg P2O5 ha-1 año-1) en suelo y transplantadas las variedades Chardonnay (experimento 1) y Pinot Noir (experimento 2). El diseño experimental fue de bloques completos al azar, con tres repeticiones, y diez plantas. Fueron evaluados los contenidos de P en el suelo, en hojas, altura de plantas, diámetro del tallo y materia seca del material podado durante las cosechas del 2011/12 a 2014/15. La adición de dosis crecientes de P incrementó el contenido de P disponible en el suelo después de los tres años del establecimiento de los experimentos cuando las dosis fueron superiores de 40 y 60 de P2O5 ha-1 en las variedades Pinot Noir y Chardonnay, respectivamente. En todos los estados los contenidos en P en suelo, fueron suficientes, por lo tanto, el crecimiento y contenido de P en las Hojas, no fueron afectadas.

RESUMO: O manejo de plantas espontâneas em pomares de macieira (Malus domestica) pode afetar a lixiviação de nitrogênio (N) do solo. O trabalho objetivou avaliar a lixiviação de formas de N em solo sob pomar de macieiras com diferentes manejos de plantas espontâneas. O experimento foi conduzido em um pomar de macieira implantado em 2008. Em outubro de 2011, foram selecionadas 80 plantas e implantados os tratamentos: sem manejo das plantas espontâneas (SM); dessecamento das plantas espontâneas na linha de plantio com uso de herbicidas (DL) e roçada mecânica das plantas espontâneas na linha de plantio (RL). Nas safras 2011/2012, 2012/2013 e 2013/2014 foi avaliada a produtividade. Em maio de 2012 foram instalados lisímetros com cápsula porosa nos tratamentos SM, DL e RL; e na solução coletada a 0,20m em dezesseis épocas foram analisados NH4 +-N e NO3 --N, e calculado o teor de N-mineral. Os maiores teores de NO3 --N e mineral-N ocorreram na solução no solo com DL, o que aumenta a disponibilidade do nutriente às macieiras, mas também potencializa as perdas. O manejo de plantas espontâneas e o fluxo de N na solução não afetaram a produtividade de maçã.

ABSTRACT: Weed management in apple orchards (Malus domestica) can affect the leaching of nitrogen (N) in soil. The study aimed to evaluate the potential leaching of N forms in soil of an apple orchard with different weed management treatments. The experiment was conducted in an apple orchard implanted in 2008. In October 2011, 80 plants were selected and the following treatments were implemented: no weed management (NM), desiccation of weeds on the tree row with herbicide use (DR) and mechanical mowing of weeds on the tree row (MR). Yield was evaluated in the 2011/2012, 2012/2013 and 2013/2014 crop seasons. In May 2012 porous cup lysimeters were installed in the NM, DR and MR treatments. In the solution collected at 0.20m, NH4 +-N and NO3 --N were analyzed sixteen times and mineral N concentration was calculated. The highest concentrations of NO3 --N and mineral N occurred in soil solution with DR, which increases availability of the nutrient to apple trees, but also enhances the potential losses. Weed management and N flow in the solution did not affect apple yield.

Resumo: O objetivo deste trabalho foi avaliar o efeito das aplicações de dejetos líquidos de suínos no teor de Al trocável, na atividade e na distribuição das espécies químicas de Al na solução do solo. Amostras de um Argissolo foram obtidas de um experimento conduzido durante oito anos, com 19 aplicações de 0, 20, 40 e 80 m3 ha-1 de dejetos líquidos de suínos. As amostras de solo foram coletadas em camadas, para determinação do Al trocável e extração da solução do solo pelo método da pasta de saturação. Na solução, foram determinados as concentrações dos principais cátions e ânions, o carbono orgânico dissolvido e o pH. A distribuição das espécies químicas de Al e a atividade da espécie Al3+ foram obtidas pelo programa Visual Minteq. A aplicação de dejetos líquidos de suínos não altera o teor de Al trocável no solo e a atividade de Al3+ na solução do solo. A baixa atividade de Al3+ nas camadas superficiais não apresenta risco de toxidez para a maioria das culturas. A adição de dejetos líquidos de suínos aumenta a proporção das espécies AlHPO4 + e AlSO4 + na solução do solo, por incrementar a concentração de ligantes inorgânicos solúveis.

Abstract: The objective of this work was to evaluate the effect of pig slurry applications on the exchangeable Al content, activity, and distribution of Al chemical species in the soil solution. Soil samples of a Typic Hapludalf were obtained from an experiment conducted during eight years, with 19 applications of 0, 20, 40, and 80 m3 ha-1 pig slurry. The soil samples were collected in stratified layers to determine exchangeable Al content and to extract the soil solution by the saturated soil paste method. The concentrations of the main cations and anions, dissolved organic carbon, and pH in the soil solution were determined. The distribution of the chemical species of Al and the activity of the Al3+ species were obtained through the Visual Minteq software. The application of pig slurry does not change the exchangeable Al content in the soil and the Al3+ activity in the soil solution. The low Al3+ activity in the topsoil layers does not present risk of toxicity for most crops. Pig slurry applications increase the proportion of the AlHPO4 + and AlSO4 + species in the soil solution, by increasing the concentration of soluble inorganic ligands.

ABSTRACT Phosphorus (P) applications at dosages higher than the necessary may cause P buildup in the soil labile fractions and, consequently, pollution of water sources. This study aimed to assess accumulation of P fractions as well as the parameters of adsorption isotherms in soil profiles having a history of application of phosphate fertilizers and cultivated with apple trees. Soil samples were collected from an area with no history of cultivation and 2 apple orchards cultivated for 8 (P8) and 18 (P18) years, in the city of Urubici, Santa Catarina, southern Brazil. Soil samples were air dried, sieved in a 2-mm mesh, and subjected to chemical analyses (P adsorption) besides P chemical fractionation. Applications of phosphate fertilizers to the orchards, particularly P18, increased the inorganic and organic concentrations of P in all fractions. The distribution of P in organic and inorganic forms in the planted soils was similar to the distribution observed for the native vegetation soil. The highest inorganic labile P fraction, the lowest maximum P adsorption capacity, and the highest equilibrium concentration values at P18 are indicators of a higher environmental pollution risk of the orchards soils compared to the native soil.

Resumo: O objetivo deste trabalho foi avaliar as formas de fósforo em folhas e suas relações com a composição do mosto e a produção em videiras cultivadas em um Argissolo Vermelho distrófico típico, com diferentes teores de P disponível. Dois experimentos foram realizados com cultivares de Vitis vinifera, um com uvas 'Tannat' e outro com 'Cabernet Franc'. O experimento 1 consistiu de dois vinhedos de 'Tannat', com os seguintes teores de fósforo no solo: V1, 11,8 mg kg-1 de P; e V2, 34,6 mg kg-1 de P. O experimento 2 consistiu de dois vinhedos de 'Cabernet Franc', com os seguintes teores de fósforo no solo: V1, 16,0 mg kg-1 de P; e V2, 37,0 mg kg-1 de P. Coletaram-se as folhas ao florescimento (FL) e ao início da maturação (IM), e, após a preparação delas, analisaram-se as formas de P. A produtividade e a composição do mosto foram avaliadas. A maior produtividade foi observada no V2 do experimento 1 e no V2 do experimento 2. O teor total de P e suas formas nas folhas ao FL e IM não apresentam relação com os parâmetros produtivos; no entanto, o teor de P total nas folhas apresenta relação com o teor de antocianinas no mosto de videiras 'Tannat'. Portanto, o fracionamento de P nas folhas não prediz a produtividade da videira nem a composição do mosto.

Abstract: The objective of this work was to evaluate phosphorus forms in grape leaves and their relationships with must composition and yield in grapevines grown in a Typic Hapludalf with different available P contents. Two experiments were carried out with Vitis viniferacultivars, one with 'Tannat' and the other with 'Cabernet Franc' grapes. Experiment 1 consisted of two vineyards of 'Tannat', with the following P content in the soil: V1, 11.8 mg kg-1 P; and V2, 34.6 mg kg-1 P. Experiment 2 consisted of two vineyards of 'Cabernet Franc', with the following P content in the soil: V1, 16.0 mg kg-1 P; and V2, 37.0 mg kg-1 P. Leaves were collected at flowering (FL) and veraison (V), and, after their preparation, P forms were evaluated. Yield and must composition were assessed. The highest yield was observed in V2 of experiment 1 and in V2 of experiment 2. Total P content and P forms in leaves at FL and V have no relationship with yield parameters; however, total P content in leaves has a relationship with anthocyanin content in the must of 'Tannat' grapevines. Therefore, P fractionation in leaves predicts neither grapevine yield nor must composition.

ABSTRACT Applications for long periods of organic animal waste as a source of nutrients to crops can contaminate the soil and the surface and subsurface waters. This study aimed to evaluate how the utilization of organic waste, after successive applications, affects P fractions in the soil and consequently environmental contamination. In an experiment conducted for 8 years, totaling 12 applications with pig slurry manure (PSM), liquid cattle manure (LCM), swine deep bedding (SDB), and mineral fertilizer (NPK), 460; 505; 1.111; and 535 kg P∙ha−1 were added to the soil through PSM, LCM, SDB, and NPK, respectively. In September 2012, soil samples were collected in the layers 0.0 – 0.04; 0.04 – 0.08; 0.08 – 0.12; 0.12 – 0.16; 0.16 – 0.20 m, prepared and subjected to a sequential chemical fractionation of P. The 12 applications of pig slurry, liquid cattle manure, swine deep bedding and NPK fertilizer for eight years (urea + triple superphosphate + potassium chloride) provided accumulations of P fractions in the soil that were above the necessary for a proper nutrition to plants considering the available P fractions (AER + organic NaHCO3 + inorganic NaHCO3) in the soil. In addition, they showed that the use of swine deep bedding, followed by pig slurry, means higher levels of available P fractions in the soil. In this sense, the establishment of a dosage of organic waste to meet the nitrogen need of crops is not an environmentally appropriate parameter. It may cause P accumulation in the soil, increased absorption ability of plants and soil support, contributing to environmental contamination.

ABSTRACT Nitrogen derived from cover crop residues may contribute to the nutrition of onion grown under minimum tillage (MT) and cultivated in rotation. The aim of this study was to evaluate the N transferred from different cover crop residues to the onion crop cultivated under MT in southern Brazil. In June 2014, oilseed radish, black oat, and oilseed radish + black oat residues labeled with 15N were deposited on the soil surface before transplanting onions. During the growth season and at harvest, young expanded onion leaves, complete plants, and samples from different soil layers were collected and analyzed for recovery of 15N-labeled residue. Oilseed radish decomposed faster than other residues and 4 % of residue N was recovered in leaves and bulbs at harvest, but in general, N in plant organs was derived from sources other than the cover crop residues. In addition, leaf N was in the proper range for all treatments and was adequately mobilized to the bases for bulbing. The N derived from decomposing residues contributed little to onion development and the use of these plants should be chosen based on their advantages for physical and biological soil quality.

RESUMO: O potássio (K) é um dos macronutrientes exigidos em maior quantidade pela videira, porém o seu incremento no solo pode não aumentar a produção de uva, mas pode modificar a sua composição. O trabalho objetivou avaliar a interferência do teor de K trocável no solo sobre o seu teor na folha, produção e composição do mosto da cv. 'Cabernet Sauvignon'. Em setembro de 2011, em Santana do Livramento (RS), foram selecionados cinco vinhedos com níveis crescentes de K trocável no solo. Nas safras 2012/13 e 2013/14, foi avaliada a produção de uva, os componentes de produção, o teor total de K nas folhas no pleno florescimento e na mudança da cor das bagas. No mosto, foram avaliados os valores de sólidos solúveis totais (SST), pH, a acidez total titulável (ATT) e os totais de polifenóis e antocianinas. O incremento do teor de K trocável no solo aumentou o K total nas folhas no pleno florescimento, nas bagas e a composição do mosto, mas não afetou a produção de uva da cv. 'Cabernet Sauvignon'.

ABSTRACT: Content of exchangeable potassium (K) in t soil may influence on its content in grapevines leaves, grape yield, as well as, in must composition. The study aimed to assess the interference of exchangeable K content in the soil on its leaf content, production and must composition of 'Cabernet Sauvignon' cultivar. In September 2011, in Santana do Livramento (RS) five vineyards with increasing levels of exchangeable K in the soil were selected. In the 2012/13 and 2013/14 harvests, the grape yield, yield components, total K content in the leaves in full bloom and berries veraison were evaluated. Values of total soluble sugar (TSS), pH, total titratable acidity (TTA), total polyphenols and anthocyanins were evaluated in the must. Exchangeable K content increase in soil with sandy surface texture increased its content in leaves collected during full flowering and in berries and must pH; however, it did not affect production of the 'Cabernet Sauvignon'.

Abstract High copper (Cu) levels in uprooted old vineyard soils may cause toxicity in transplanted young vines, although such toxicity may be reduced by inoculating plants with arbuscular mycorrhizal fungi (AMF). The objective of this study was to evaluate the effects of AMF on the plant growth, chlorophyll contents, mycorrhizal colonization, and Cu and phosphorus (P) absorption in young vines cultivated in a vineyard soil contaminated by Cu. Commercial vineyard soil with high Cu levels was placed in plastic tubes and transplanted with young vines, which were inoculated with six AMF species (Dentiscutata heterogama, Gigaspora gigantea, Acaulospora morrowiae, A. colombiana, Rhizophagus clarus, R. irregularis) and a control treatment on randomized blocks with 12 replicates. After 130 days, the mycorrhizal colonization, root and shoot dry matter (DM), height increment, P and Cu absorption, and chlorophyll contents were evaluated. The height increment, shoot DM and chlorophyll contents were not promoted by AMF, although the root DM was increased by R. clarus and R. irregularis, which had the greatest mycorrhizal colonization and P uptake. AMF increased Cu absorption but decreased its transport to shoots. Thus, AMF species, particularly R. clarus and R. irregularis, contribute to the establishment of young vines exposed to high Cu levels.

A decomposição das plantas de cobertura de inverno, depositadas sobre o solo, podem aumentar a disponibilidade de formas de nitrogênio (N), durante o ciclo da cebola. O trabalho objetivou avaliar a mineralização de N da massa de plantas de cobertura, solteiras e consorciadas, em um solo com histórico de cultivo de cebola. Porções de solo foram coletadas, preparadas, acondicionadas em recipientes de acrílico. Matéria seca de aveia preta, centeio, nabo-forrageiro, aveia preta+nabo-forrageiro e centeio+nabo-forrageiro, foram adicionadas sobre a superfície do solo e incubadas por 90 dias. No tempo zero e aos 18, 36, 54, 72 e 90 dias após a incubação (DAI), as porções de solo foram amostradas e determinados os teores de N total, N-NO3- e N-NH4+ de cada uma. Calcularam-se os valores de N mineral, N mineral líquido, N mineralizado e N total-N mineralizado. Os maiores teores de N-NH4+ foram observados nas porções de solo com a deposição de massa de nabo-forrageiro e do consórcio centeio+nabo-forrageiro. Os maiores teores de N-NO3- e N-mineral dos 36 até os 90 DAI e de N-mineralizado dos 18 até os 92 DAI foram observados nas porções de solo com a deposição de massa de centeio + nabo-forrageiro. A taxa de mineralização foi positiva em todas as amostras do solo com deposição de massa de centeio e nabo-forrageiro, e dos consórcios aveia preta+nabo-forrageiro e centeio+nabo-forrageiro e negativa aos 18 e 72 DAI, nas porções de solo com deposição de massa de aveia. Os resíduos de nabo-forrageiro e do consórcio centeio+nabo-forrageiro apresentaram o maior potencial de mineralização.

The decomposition of winter cover plants on the soil surface can increase nitrogen (N) availability during the onion crop cycle. The objective of this study was to evaluate N mineralization of cover plant litter, intercropeed or not, in a soil with a history of onion plantation. Soil was collected, prepared, placed in acrylic containers. Dry mass of black oats, rye, oilseed radish, black oats + oilseed radish and rye + oilseed radish were added into soil surface and incubated for 90 days. Soil was sampled and contents of total N, NO3--N and NH4+-N were determined at time zero and at 18, 36, 54, 72 and 90 days after incubation. Values of mineral N, net mineral N, mineralized N and total N - mineralized N were calculated. The highest contents of NH4+-N were found in the soil with the deposition of oilseed radish litter and rye + oilseed radish intercrop. The greatest contents of NO3--N and mineral N from 36 to 90 days after incubation and of mineralized N from 18 to 92 DAI were found in the soil with the rye + oilseed radish litter deposition. Mineralization rate was positive in all soil samples with deposition of rye and oilseed radish litter, and black oats + oilseed radish and rye + oilseed radish intercrops, and negative on 18 and 72 days after incubation in the soil with deposition of black oats litter. The oilseed radish litter and rye + oilseed radish intercrop displayed the greatest potential for mineralization.

O objetivo deste trabalho foi avaliar os atributos microbiológicos e químicos de um solo com histórico de sete anos de aplicação de ureia e dejetos de suínos. No período de outubro de 2008 a outubro de 2009, foram coletadas amostras de solo na camada de 0-10 cm, as quais foram submetidas aos tratamentos: controle, sem aplicação de ureia ou dejetos; e com a aplicação de ureia, dejetos líquidos e cama sobreposta de suínos em duas doses, para suprir uma ou duas vezes a dose de N recomendada para a sucessão milho (Zea mays)/aveia‑preta (Avena strigosa). O carbono da biomassa microbiana (C‑BM) e a respiração basal (C‑CO2) foram analisados, e o coeficiente metabólico (qCO2) e o microbiano (qmic) foram calculados com os dados obtidos. Também foram determinados matéria orgânica, pH em água, P e K disponíveis, e Ca e Mg trocáveis. A aplicação do dobro da dose de cama sobreposta de suínos aumenta os valores de C‑BM e C‑CO2. O qmic e o qCO2 são pouco afetados pela aplicação de dejetos de suínos. A aplicação do dobro da dose de cama sobreposta de suínos aumenta os valores de pH em água e os teores de P disponível e de Ca e Mg trocáveis no solo.

The objective of this work was to evaluate the microbiological and chemical attributes of a soil with a seven‑year history of urea and swine manure application. In the period from October 2008 to October 2009, soil samples were collected in the 0-10 cm layer and were subjected to the treatments: control, without application of urea or manure; and with the application of urea, pig slurry, and deep pig litter in two doses, in order to supply one or two times the recommended N doses for the maize (Zea mays)/black oat (Avena strigosa) crop succession. The carbon of the microbial biomass (MB‑C) and the basal respiration (C‑CO2) were analyzed, and the metabolic (qCO2) and microbial quotient (qmic) were calculated with the obtained data. Organic matter, pH in water, available P and K, and exchangeable Ca and Mg were also determined. The application of twice the dose of deep pig litter increases the MB‑C and C‑CO2 values. The qmic and qCO2 are little affected by the application of swine manure. The application of twice the dose of deep pig litter increases the values of pH in water and the contents of available P and of exchangeable Ca and Mg in the soil.

As aplicações sucessivas de dejetos líquidos e cama sobreposta de suínos podem provocar o acúmulo de frações de cobre (Cu) e zinco (Zn) no perfil do solo. Este trabalho objetivou avaliar as formas e o acúmulo de Cu e Zn em um Argissolo Vermelho com histórico de aplicação de dejetos líquidos e cama sobreposta de suínos. Em março de 2010, após oito anos da instalação de um experimento localizado no município de Braço do Norte, Santa Catarina, SC, sobre um Argissolo Vermelho, foram coletadas amostras de solo nas camadas de 0-2,5; 2,5-5,0; 5-10; e 10-15 cm, em tratamentos sem a aplicação de dejetos e com a aplicação de dejetos líquidos de suínos e de cama sobreposta de suínos em duas doses para suprir uma e duas vezes a necessidade de N para a sucessão milho e aveia preta. O solo foi seco, moído em gral de pedra ágata e submetido à análise dos teores de Cu e Zn por EDTA 0,01 mol L-1 e ao fracionamento químico de Cu e Zn. A aplicação de cama sobreposta de suínos e dejetos líquidos de suínos aumentou os teores de Cu e Zn disponíveis na camada superficial do solo; entretanto, quando se aplicaram a dose (equivalente a 90 kg ha-1 de N) e o dobro da dose de cama sobreposta de suínos e o dobro da dose de dejetos líquidos de suínos o Cu e o Zn migraram até 15 cm de profundidade. O Cu no solo é acumulado especialmente na fração orgânica e residual e o Zn, preferencialmente na fração ligada aos argilominerais, especialmente nas camadas mais superficiais.

Successive applications of pig slurry and pig deep litter may lead to an accumulation of copper (Cu) and zinc (Zn) fractions in the soil profile. The objective of this study was to evaluate the Cu and Zn forms and accumulation in a Sandy Typic Hapludalf soil after long-term application of pig slurry and deep litter. In March 2010, eight years after initiating an experiment in Braço do Norte, Santa Catarina (SC), Brazil, on a Sandy Typic Hapludalf soil, soil samples were collected from the 0-2.5, 2.5-5.0, 5-10 and 10-15 cm layers in treatments consisting of no manure application (control) and with applications of pig slurry and deep litter at two levels: the single and double rate of N requirement for maize and black oat succession. The soil was dried, ground in an agate mortar and analyzed for Cu and Zn contents by 0.01 mol L-1 EDTA and chemically fractionated to determine Cu and Zn. The applications of Pig deep litter and slurry at doses equivalent to 90 kg ha-1 N increased the contents of available Cu and Zn in the surface soil layer, if the double of this dose was applied in pig deep litter or double this dose in pig slurry, Cu and Zn migrated to a depth of 15 cm. Copper is accumulated mainly in the organic and residual fractions, and zinc preferentially in the fraction linked to clay minerals, especially in the surface soil layers.